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bromophenol blue loading dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bromophenol blue loading dye
    Bromophenol Blue Loading Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bromophenol+blue+loading+dye/Bromophenol+Blue/pmc13054239-125-15-28
    Average 97 stars, based on 1 article reviews
    bromophenol blue loading dye - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Structural diversity and evolution of the Rf-1 locus in the genus Oryza.
    Article Snippet: Thirty-five PCR cycles, each consisting of 10 s of denaturation at 941C, 30 s of annealing at 551C and 2 or 3 min of polymerization at 721C, were performed using a Thermal Cycler 9600 or Name Sequence a 50-GTAAAGAACAAAGCTTCTTCAGAC-30 b 50-GCCTCGATCTACGGCTTGAA-30 c 50-CGATCTCTCATTCTCTCCAC-30 d 50-AGCTAAAGTTTCAGCAGAGG-30 e 50-GGCTCTCAAGGCTAAGCTTG-30 f 50-GTAACTAGTCTCAGTACCTG-30 g 50-CCCTTCTCTCCCACCGTAAA-30 h 50-GACAGGGGTTGCTAGAAGAA-30 i 50-TCCCTCCTCTAATAGGACTG-30 j 50-AGCTAAAGTTTCAGCAGAGG-30 k 50-AACTGCGCAAGAGATCGATC-30 l 50-GGGAGGGTTATAGAATCTGG-30 m 50-CCTCGAGATGCGTGATTA-30 n 50-GAGCCAAATAAGCAGTTGTC-30 o 50-CACAGATAGGAACACCAAGC-30 p 50-ATTAGAGGAGGGATCTTCTC-30 q 50-GATTGATACACACAAATGAATCC-30 r 50-CTCTCCGTATCAGCTGCGTC-30 s 50-GTAGGTCACAACATTTGGCG-30 Heredity 9700 (Perkin-Elmer). .. The PCR products were mixed with bromophenol blue loading dye and were analyzed by electrophoresis on 1% agarose gels (Invitrogen, Calsbad, CA, USA) using 1 TBE (tris-borate-ethylenediamine tetraacetic acid) buffer at room temperature. ..

    Article Title: Transplacental Transmission of Cutaneous Leishmania mexicana Strain in BALB/c Mice
    Article Snippet: A 2.5% ultrapure agarose gel (Invitrogen, Sao Paulo, Brazil) was prepared and diluted in Tris-Acetate-EDTA (TAE) buffer 1× (containing 40 mM Tris·acetate and 1 mM EDTA, pH 8.3), which was boiled until complete dissolution was achieved. .. The samples were mixed with 1 μL bromophenol blue loading dye (10× BlueJuice Gel Loading Buffer; Invitrogen, Sao Paulo, Brazil), and a molecular weight marker (50-bp DNA ladder; Invitrogen, Sao Paulo, Brazil) was loaded in the first well; 5-μL aliquots were taken from each PCR sample and added to the wells, and the gel was run at 60 V for 2 hours. .. The samples were then stained with 0.5 μg/mL ethidium bromide (Ultrapure Ethidium Bromide; Invitrogen, Sao Paulo, Brazil) for 20 minutes and washed with bidistilled water before observation using a gel imaging system (AlphaImager HP system, Cell Biosciences, Inc., Santa Clara, CA).

    Electrophoresis:

    Article Title: Structural diversity and evolution of the Rf-1 locus in the genus Oryza.
    Article Snippet: Thirty-five PCR cycles, each consisting of 10 s of denaturation at 941C, 30 s of annealing at 551C and 2 or 3 min of polymerization at 721C, were performed using a Thermal Cycler 9600 or Name Sequence a 50-GTAAAGAACAAAGCTTCTTCAGAC-30 b 50-GCCTCGATCTACGGCTTGAA-30 c 50-CGATCTCTCATTCTCTCCAC-30 d 50-AGCTAAAGTTTCAGCAGAGG-30 e 50-GGCTCTCAAGGCTAAGCTTG-30 f 50-GTAACTAGTCTCAGTACCTG-30 g 50-CCCTTCTCTCCCACCGTAAA-30 h 50-GACAGGGGTTGCTAGAAGAA-30 i 50-TCCCTCCTCTAATAGGACTG-30 j 50-AGCTAAAGTTTCAGCAGAGG-30 k 50-AACTGCGCAAGAGATCGATC-30 l 50-GGGAGGGTTATAGAATCTGG-30 m 50-CCTCGAGATGCGTGATTA-30 n 50-GAGCCAAATAAGCAGTTGTC-30 o 50-CACAGATAGGAACACCAAGC-30 p 50-ATTAGAGGAGGGATCTTCTC-30 q 50-GATTGATACACACAAATGAATCC-30 r 50-CTCTCCGTATCAGCTGCGTC-30 s 50-GTAGGTCACAACATTTGGCG-30 Heredity 9700 (Perkin-Elmer). .. The PCR products were mixed with bromophenol blue loading dye and were analyzed by electrophoresis on 1% agarose gels (Invitrogen, Calsbad, CA, USA) using 1 TBE (tris-borate-ethylenediamine tetraacetic acid) buffer at room temperature. ..

    Amplification:

    Article Title: Swabbing as a Non‐Lethal DNA Collection Method for Earthworm Barcoding: Performance and Citizen Science Perspectives
    Article Snippet: Amplicons were monitored by running a 1% agarose gel electrophoresis and stained with ROTI GelStain (0.05 μL/mL of gel; Carl ROTH). .. Each well in the gel held 3 μL of amplicon and 2 μL of 0.25% bromophenol blue loading dye, with 3 μL of 100 bp DNA ladder (GeneRuler, Thermo Fisher Scientific Inc.) pipetted into the first lane. .. Enzymatic clean‐up of successful amplicons, as indicated by a positive gel electrophoresis result, was performed by reacting 6 μL of the amplicon with 0.5 μL Exonuclease I (20 U/μL; Thermo Fisher Scientific Inc.) and 1 μL Alkaline Phosphatase (1 U/μL; Fast AP, Thermo Fisher Scientific Inc.).

    Molecular Weight:

    Article Title: Transplacental Transmission of Cutaneous Leishmania mexicana Strain in BALB/c Mice
    Article Snippet: A 2.5% ultrapure agarose gel (Invitrogen, Sao Paulo, Brazil) was prepared and diluted in Tris-Acetate-EDTA (TAE) buffer 1× (containing 40 mM Tris·acetate and 1 mM EDTA, pH 8.3), which was boiled until complete dissolution was achieved. .. The samples were mixed with 1 μL bromophenol blue loading dye (10× BlueJuice Gel Loading Buffer; Invitrogen, Sao Paulo, Brazil), and a molecular weight marker (50-bp DNA ladder; Invitrogen, Sao Paulo, Brazil) was loaded in the first well; 5-μL aliquots were taken from each PCR sample and added to the wells, and the gel was run at 60 V for 2 hours. .. The samples were then stained with 0.5 μg/mL ethidium bromide (Ultrapure Ethidium Bromide; Invitrogen, Sao Paulo, Brazil) for 20 minutes and washed with bidistilled water before observation using a gel imaging system (AlphaImager HP system, Cell Biosciences, Inc., Santa Clara, CA).

    Marker:

    Article Title: Transplacental Transmission of Cutaneous Leishmania mexicana Strain in BALB/c Mice
    Article Snippet: A 2.5% ultrapure agarose gel (Invitrogen, Sao Paulo, Brazil) was prepared and diluted in Tris-Acetate-EDTA (TAE) buffer 1× (containing 40 mM Tris·acetate and 1 mM EDTA, pH 8.3), which was boiled until complete dissolution was achieved. .. The samples were mixed with 1 μL bromophenol blue loading dye (10× BlueJuice Gel Loading Buffer; Invitrogen, Sao Paulo, Brazil), and a molecular weight marker (50-bp DNA ladder; Invitrogen, Sao Paulo, Brazil) was loaded in the first well; 5-μL aliquots were taken from each PCR sample and added to the wells, and the gel was run at 60 V for 2 hours. .. The samples were then stained with 0.5 μg/mL ethidium bromide (Ultrapure Ethidium Bromide; Invitrogen, Sao Paulo, Brazil) for 20 minutes and washed with bidistilled water before observation using a gel imaging system (AlphaImager HP system, Cell Biosciences, Inc., Santa Clara, CA).



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